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KMID : 0380219940270060473
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 6 p.473 ~ p.478
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Determination of the Antioxidant Activity of Vitamins C and E According to Lipi dPhysical State Using High Performance Liquid Chromatography with Chemiluminescence Detection
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Abstract
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Abstract:
@EN The oxidation of phosphatidylcholine (PC) was initiated with 2,2'-azobis(2-amidinopropane)dihydrochloride(AAPH), a water-soluble radical initiator, and 2,2'-azobis(2,4-dimethylvaleronitrile)(AMVN), a lipidsoluble radical initiator, in
liposomal
suspension and homogeneous organic solution, respectively. Generation of phosphatidylcholine hydroperoxide (PCOOH) was measured by high performance liquid chromatography (HPLC) with a chemiluminescence detector. The antioxidant activities of
vitamins E
(V.E), vitamin C(V.C) and a mixture of vitamin E and C(V.E+V.C) were examined based on lipid physical state. To investigate the relationship between activity and antioxidant location, antioxidants were added before and after the formation of
liposomes,
respectively. The antioxidant activities of the vitamins in homogeneous organic solutions(i.e., methanol, hexane) were compared. When vitamins E and C were added separately after the formation of liposomes they showed similar activities. Under
the
same
conditions a mixture of vitamins E and C exhibited a great antioxidant activity. The addition of the antioxidants prior to the formation of liposomes resulted in antioxidant activities in the following order.: V.E+V.C>V.C. In methanol, vitamin C
showed
a higher antioxidant activity than vitamin E plus vitamin C, and vitamin E alone did not show activity. In hexane vitamin E showed the highest activity of the molecules examined.
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KEYWORD
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